Journal: Cells

Article Title: Extracellular Vesicles from Ocular Melanoma Have Pro-Fibrotic and Pro-Angiogenic Properties on the Tumor Microenvironment

doi: 10.3390/cells11233828

Figure Lengend Snippet: UM-derived EVs increase collagen gel contraction by stellate cells. ( A ) Representative images of collagen gels (1 mg/mL) without embedded HSteCs, with embedded HSteCs not treated with EVs, and with embedded HSteCs treated during 24 h with large EVs derived from NCMs or metastatic UM cell line TJU-UM001 taken with a stereomicroscope. ( B ) Corresponding quantification of the collagen gel area (in percentage) for each condition during 48 h. Error bars indicate ±SEM ( n = 3); ** p < 0.01 and **** p < 0.0001 (two-way ANOVA with Sidak’s multiple comparisons test).

Article Snippet: After 1 h of incubation at 37 °C, the outward edge of the gel was carefully detached with a pipette tip, and phase contrast pictures were taken at 0 h, 24 h, and 48 h using a stereomicroscope (Zeiss SteREO Discovery v8 with an AxioCam ERc5s, Axio Vision software version 4.8).

Techniques: Derivative Assay